Immunoelectrophoretic analysis of the porcine zona pellucida

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1 Zona Pellucida Glycoproteins

Abbreviations: ZP, zona pellucida; ZPG, ZP glycoprotein; VE, vitelline envelope; VEG, VE glycoprotein; mZP, mouse ZP; hZP, human ZP; SS, signal sequence; CTP, C-terminal propeptide; CFCS, consensus furin cleavage-site; TMD, transmembrane domain; ZPD, ZP domain; NTS, N-terminal subdomain; CTS, C-terminal subdomain; EHP, external hydrophobic patch; IHP, internal hydrophobic patch; CP, charged pat...

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Structural Analysis of Peptide-Analogues of Human Zona Pellucida ZP1 Protein with Amyloidogenic Properties: Insights into Mammalian Zona Pellucida Formation

Zona pellucida (ZP) is an extracellular matrix surrounding and protecting mammalian and fish oocytes, which is responsible for sperm binding. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. These proteins polymerize into long interconnected filaments, through a common structural unit, known as the ZP domain, which consists of two domains, ZP-N and ZP-C. ZP is re...

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Three-dimensional structure of the zona pellucida.

The zona pellucida is the extracellular coat that surrounds the mammalian oocyte. It forms a spherical shell of remarkably uniform thickness (5-10 microns in eutherian mammals). The mouse is currently the largest source of data on the zona pellucida and this review is built largely on these data. The zona pellucida is composed of three proteins in both mice and humans: ZP1, ZP2 and ZP3. These p...

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Caprine acrosin. Purification, characterization and proteolysis of the porcine zona pellucida.

Acrosin purified from an acidic extract of ejaculated goat spermatozoa migrated as a single 42,000-Mr band in SDS/polyacrylamide-gel electrophoresis. Reduction and alkylation of caprine acrosin produced two polypeptides, one of Mr 40,000 (heavy chain) and the other of Mr 3700 (light chain). The light chain purified by reversed-phase h.p.l.c. was a glycosylated octadecapeptide with an amino acid...

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Mammalian fertilization: the egg's multifunctional zona pellucida.

The zona pellucida (ZP) is a specialized extracellular coat that surrounds the plasma membrane of mammalian eggs. Its presence is essential for successful completion of oogenesis, fertilization and preimplantation development. The ZP is composed of only a few glycoproteins which are organized into long crosslinked fibrils that constitute the extracellular coat. A hallmark of ZP glycoproteins is...

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ژورنال

عنوان ژورنال: Reproduction

سال: 1983

ISSN: 1470-1626,1741-7899

DOI: 10.1530/jrf.0.0680021